Mouse Anti-KIT Recombinant Antibody (clone 79D)
CAT#: PABL-642
Recombinant Mouse Antibody (79D) is capable of binding to Kit. The inhibitory KIT antibodies that bind to the membrane-proximal Ig-like D4 of KIT with significant overlap with an epitope in D4 that mediates homotypic interactions essential for KIT activation.
Published Data
Tested Data
Gene Expression
IB
Figure 1 WT KIT activation is inhibited by anti-D4 antibodies.
NIH 3T3 cells expressing WT KIT were incubated for 5 h with the indicated concentrations (Upper) of Fab79D following stimulation with 25 ng/mL of SCF for 5 minat 37 °C. Lysates of unstimulated or SCF-stimulated cells were subjected to immunoprecipitation (IP) with anti-KIT antibodies followed by SDS/PAGE and immunoblotting (IB) with anti-KIT or anti-phosphotyrosine (pY) anti-bodies.
Reshetnyak, A. V., Nelson, B., Shi, X., Boggon, T. J., Pavlenco, A., Mandel-Bausch, E. M., ... & Schlessinger, J. (2013). Structural basis for KIT receptor tyrosine kinase inhibition by antibodies targeting the D4 membrane-proximal region. Proceedings of the National Academy of Sciences, 110(44), 17832-17837.
Inhib
Figure 2 WT KIT activation is inhibited by anti-D4 antibodies.
Ba/F3 cells expressing WT KIT were plated in six-well platesand grown in the presence of 250 ng/mL SCF and different concentrations as indicated of Fab 79D.
Reshetnyak, A. V., Nelson, B., Shi, X., Boggon, T. J., Pavlenco, A., Mandel-Bausch, E. M., ... & Schlessinger, J. (2013). Structural basis for KIT receptor tyrosine kinase inhibition by antibodies targeting the D4 membrane-proximal region. Proceedings of the National Academy of Sciences, 110(44), 17832-17837.
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SDS-PAGE
Figure 1 Mouse Anti-KIT Recombinant Antibody (PABL-642) in SDS-PAGE
SDS-PAGE analysis of PABL-642 in non-reduced (Lane 1) and reduced (Lane 2) conditions.
SEC-HPLC
Figure 2 Mouse Anti-KIT Recombinant Antibody (PABL-642) in HPLC
The purity of PABL-642 was greater than 95% as determined by SEC-HPLC.
WB
Figure 3 Mouse Anti-KIT Recombinant Antibody (PABL-642) in WB
Western blot analysis of PABL-642 was performed by loading c-Kit Protein, Human, Recombinant (His Tag).
PABL-642 incubation concentration: 2ng/μL.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
Lane 1: Reducing Antigen (0.1μg)
Lane 2: Reducing Antigen (0.3μg)
Lane 3: Reducing Antigen (0.6μg)
ELISA
Figure 4 Mouse Anti-KIT Recombinant Antibody (PABL-642) in ELISA
ELISA analysis of PABL-642 was performed by coating with c-Kit Protein, Human, Recombinant (His Tag).
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
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Subcellular Location
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/73252/1469_D7_6_selected.jpg
Normal Tissue
Figure 2 High expression in breast
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4471/ihc_selected.jpg
Normal Tissue
Figure 3 High expression in lung.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3288/8181_A_1_4_val_selected.jpg
Normal Tissue
Figure 4 Skin
Melanocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3288/8181_B_8_1.jpg
RNA Expression
Figure 5 RNA cell line category: Group enriched (HEL, HMC-1)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000157404-KIT
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Specifications
- Immunogen
- Human v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG
- Specificity
- Tested positive against Kit
- Species Reactivity
- Human
- Clone
- 79D
- Applications
- WB, FuncS
Product Property
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term.
Target
- Alternative Names
- KIT; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; PBT; SCFR; C-Kit; CD117; maststem cell growth factor receptor Kit; p145 c-kit; proto-oncogene c-Kit; piebald trait protein; soluble KIT variant 1; tyrosine-protein kinase Kit; proto-oncogene tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein
- Gene ID
- 3815
- UniProt ID
- A0A024RDA0
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(Creative Biolabs Cat# PABL-642, RRID: AB_3111666)
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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